Analysis of Tetracycline and Miconazole
Effect of 719D External Needle Wash on Injection to Injection Carryover

Tetraycline and Miconazole were selected for the study because they exhibit unusually high levels of carryover under conventional needle wash procedures. Removal of these compounds to acceptable levels is an indication of the effectiveness of the 719D External Needle Wash under severe conditions.

 

Typical Autosampler LC-MS Carryover Performance for Tetracycline | Injected 20 µL of 50ppm Tetracycline Standard followed by 20 µL injections of mobile phase as blank
Typical Carryover Performance of the 719D with External Needle Wash for Tetracycline

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Analysis Conditions

Analysis Conditions

Column: Michrom BioresourcesTM C-18 Magic Bullet®
Mobile Phase A: 0.1% Hepta Fluoro Butyric Acid in Acetonitrile
Mobile Phase B: 0.1% Hepta Fluoro Butyric Acid in Water
Solvent Ratios: 60% A, 40% B
Flow Rate: 0.05 mL/min
Column Temperature: 40 degrees C
Run Time: 7 minutes
Sample Size: 20uL 70/30 Methanol/Water
Rinse Solvent A: 0.1% TFA in Acetonitrile, 5 seconds
Rinse Solvent B: 50% Acetonitrile in water, 5 seconds
Rinse Evacuation: 3 seconds
Internal Rinse: 0.1% Hepta Fluoro Butyric Acid in 50% Acetonitrile 50% Water
Mass Spectrometer: Thermo FinniganTM LCQ Deca® Ion Trap
Source Type: ESI
Source Voltage: 5 kV
Source Current: 3.1 uA
Ionization: ESI
Mode: SRM MS/MS
Sheath Gas: Helium
Gas Flow Rate: 9 mL/min
Detector Mode: Positive Ion
Primary Mass: 309 Scan Range: 280.5-281.5
Blank Solvent: 70/30 Methanol/Water