Column: Michrom Bioresources^TM C-18 Magic Bullet®
Mobile Phase A: 0.1% Hepta Fluoro Butyric Acid in Acetonitrile
Mobile Phase B: 0.1% Hepta Fluoro Butyric Acid in Water
Solvent Ratios: 60% A, 40% B
Flow Rate: 0.05 mL/min
Column Temperature: 40 degrees C
Run Time: 7 minutes
Sample Size: 20uL 70/30 Methanol/Water
Rinse Solvent A: 0.1% TFA in Acetonitrile, 5 seconds
Rinse Solvent B: 50% Acetonitrile in water, 5 seconds
Rinse Evacuation: 3 seconds
Internal Rinse: 0.1% Hepta Fluoro Butyric Acid in 50% Acetonitrile 50% Water
Mass Spectrometer: Thermo Finnigan^TM LCQ Deca® Ion Trap
Source Type: ESI
Source Voltage: 5 kV
Source Current: 3.1 uA
Ionization: ESI
Mode: SRM MS/MS
Sheath Gas: Helium
Gas Flow Rate: 9 mL/min
Detector Mode: Positive Ion
Primary Mass: 309 Scan Range: 280.5-281.5
Blank Solvent: 70/30 Methanol/Water